Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses

Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses.

Seven rotavirus strains were isolated in cell cultures from the intestinal contents of piglets with diarrhea. MA104 cells with pancreatin in the cell culture medium was the host system of choice for virus isolation and replication. A cell culture immunofluorescence test in which MA104 cells were used in microtiter plates was very effective for detecting and assaying rotaviruses. A plaque reduct...

Isolation and Serotyping of Porcine Rotaviruses and Antigenic Comparison with Other Rotavirusest

very effective for detecting and assaying rotaviruses. A plaque reduction neutralization test, crossprotection studies in gnotobiotic pigs, and electrophoresis of rotaviral double-stranded RNA were used for comparing viruses. Three strains produced plaques on initial isolation attempts, replicated well in cell cultures, and were antigenically very similar. We suggest that these three strains be...

a comparison of teachers and supervisors, with respect to teacher efficacy and reflection

supervisors play an undeniable role in training teachers, before starting their professional experience by preparing them, at the initial years of their teaching by checking their work within the proper framework, and later on during their teaching by assessing their progress. but surprisingly, exploring their attributes, professional demands, and qualifications has remained a neglected theme i...

Antigenic and pathogenic relationships of three bovine rotaviruses and a porcine rotavirus.

The antigenic relationships between three bovine rotaviruses, UK, CP-1 and PP-1, and a porcine rotavirus were investigated; their pathogenicity for piglets was also assessed. After propagation and cloning in cell culture, the four viruses were used to produce convalescent and hyperimmune antisera in gnotobiotic animals. For the UK and PP-1 viruses, reciprocal virus neutralization tests gave rat...

Serotyping of human group A rotaviruses in Nara Prefecture, Japan.